Coding
Part:BBa_K3346006:Design
Designed by: Emily Laskey Group: iGEM20_Rochester (2020-10-11)
Anti-Estradiol Fab Fragment with Anionic Peptide Tag for Drug Delivery
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The fab fragment sequence was optimized for use in E. coli with alternative codons selected to remove incompatible restriction sites.
Source
This sequence comes from a murine monoclonal antibody sequence and the sequence of a synthetically produced peptide.